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xiao shu ji yin zu rapd-pcr fan ying tiao jian de you hua yu shai xuan
Author(s): 
Pages: 170-173
Year: Issue:  3
Journal: LABORATORY ANIMAL AND COMPARATIVE MEDICINE

Keyword:  随机扩增多态性分析遗传检测筛选;
Abstract: 目的筛选优化小鼠RAPD-PCR反应体系及扩增程序.方法以2条引物对4个品系的小鼠基因组DNA在各种不同条件下进行RAPD-PCR,分析比较扩增结果.结果扩增结果较好的反应体系及扩增程序如下:在25ml的反应体系中含有10×buffer2.5μl,dNTP 200mmol/L,Mg2+2.5 mmol/L,引物0.2 mmol/L,Taq酶1.25 U,扩增程序为94℃变性1 min,40℃退火1 min,72℃延伸90 s,40个循环后72℃延伸5 min.结论这一反应体系和扩增程序在我们实验室得到了较好的扩增效果.
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