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Sequencing and analysis of ob and TNF-α Gene of Bos grunniens
Author(s): 
Pages: 94-98
Year: Issue:  1
Journal: JOURNAL OF SOUTHWEST UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCE EDITION)

Keyword:  牦牛肥胖基因肿瘤坏死因子-α基因克隆测序;
Abstract: 肥胖基因(ob gene)是近年来刚被克隆的新基因,该基因的表达产物瘦蛋白(Leptin)具有调节体重、影响动物生长和繁殖率等一系列生物学功能.肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)是一种主要由活化单核巨噬细胞和T淋巴细胞产生的多效性细胞因子,对TNF-α基因的基础及应用研究已成为近年来动物抗病育种的研究热点之一.本文克隆及测序分析了牦牛上述两个基因的部分片段,结果表明:ob基因大小为1773bp,TNF-α基因为1160bp,通过genebank中的blastn比较分析牦牛ob基因和TNF-α基因与普通牛种的相应基因同源性都高达98%.
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