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Study on the concent and chromatogram of the flavones content in Crisium japanisum and Crisium segetum
Author(s): 
Pages: 90-92
Year: Issue:  2
Journal: QILU PHARMACEUTICAL AFFAIRS

Keyword:  大蓟小蓟黄酮类分光光度法反相高效液相;
Abstract: 目的对大蓟、小蓟中黄酮类成份的含量进行测定,并对其中的成分进行分离和鉴别.方法采用紫外分光光度法对大蓟、小蓟中黄酮类成分进行含量测定,并采用反相高效色谱法对其中的成分进行分离和鉴别.结果采用紫外分光光度法测定大蓟、小蓟中总黄酮含量时,芦丁对照品在 8.040~312.600μg·ml-1范围内呈良好的线性关系(r=0.9999),平均回收率分别为100.53%、99.87%;采用反相高效液相色谱法,各组分峰分离较好,特征峰明显.结论分光光度法方法简便、准确、重现性好,结合反相高效液相色谱法可以对大蓟、小蓟这两味药材进行质量控制.
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