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Simultaneous determination of five components in Cuscutae Semen from different manufactures by UPLC
Author(s): 
Pages: 632-635,640
Year: Issue:  11
Journal: Qilu Pharmaceutical Affairs

Keyword:  菟丝子超高效液相色谱含量测定;
Abstract: 目的 采用超高效液相色谱(UPLC)法同时测定市售菟丝子中5种化学成分的含量.方法 采用Agilent SB-C18(100 mm×2.1 mm,1.8μm);流动相为乙腈-0.2%甲酸水溶液进行梯度洗脱;流速:0.4 mL·min-1;柱温:35℃;分段检测波长:1~5 min,325 nm;5~12 min,260 nm;12~30 min,360 nm.结果 5种成分的检测范围分别为绿原酸:0.50~200.09 mg·L-1(r=0.9999)、金丝桃苷:0.56~226.32 mg·L-1(r=0.9999)、异槲皮苷:0.45~180.60 mg·L-1(r=0.9999)、紫云英苷:0.43~173.07 mg·L-1(r=0.9999)、山柰酚:0.43~173.28 mg·L-1(r=0.9999);平均回收率(n=9)分别为102.0%(RSD=1.56%)、99.0%(RSD=2.90%)、100.1%(RSD=3.19%)、100.2%(RSD=3.00%)、99.3%(RSD=4.25%).结论 该方法简便、可靠,可用于菟丝子的质量评价,为进一步提高和完善菟丝子质量标准提供参考.
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