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Determination of five antimicrobial agents in Tobramycin Eye Drops by HPLC
Author(s): 
Pages: 464-467,473
Year: Issue:  8
Journal: Qilu Pharmaceutical Affairs

Keyword:  高效液相色谱法妥布霉素滴眼液抑菌剂羟苯乙酯硫柳汞羟苯丙酯苯扎溴铵苯扎氯铵;
Abstract: 目的 建立同时测定妥布霉素滴眼液中5种抑菌剂(羟苯乙酯、硫柳汞、羟苯丙酯、苯扎溴铵和苯扎氯铵)的高效液相色谱(HPLC)方法 .方法采用Agilent Zorbax SB-C18(4.6 mm×150 mm,5μm)色谱柱,流动相A为1%三乙胺(磷酸调节pH值至3.5±0.05);流动相B为甲醇,梯度洗脱,流速为1.0 mL·min-1,柱温35℃,检测波长为214 nm.结果 羟苯乙酯、硫柳汞、羟苯丙酯、苯扎溴铵/苯扎氯铵各峰均分离良好;羟苯乙酯在0.77~192.5μg·mL-1(r=0.9995),硫柳汞在2.16~541.0μg·mL-1(r=0.9997),羟苯丙酯在0.84~209.8μg·mL-1(r=1.0000),苯扎溴铵在4.36~1090μg·mL-1(r=0.9992),苯扎氯铵(n-C12H25)在3.22~805.0μg·mL-1(r=0.9998)及苯扎氯铵(n-C14H29)在1.66~413.9μg·mL-1(r=1.0000)范围内线性关系均良好.结论 该方法准确、灵敏、简便,可用于妥布霉素滴眼液中羟苯乙酯、硫柳汞、羟丙丙酯、苯扎溴铵及苯扎氯铵5种抑菌剂的检查.
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