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Biocatalytic synthesis of β-alanine from fumaric acid by a two-enzyme system
Pages: 231-235
Year: Issue:  4
Journal: Bulletin of Fermentation Science and Technology

Keyword:  L-aspartaseL-aspartateα-decarboxylasepermeable cellimmobilization;
Abstract: L-Aspartase(AspAT) and L-aspartate α-decarboxylase(PanD) catalyzed transformation of fumaric acid and ammonia is an important approach for industrial production ofβ-alanine(β-Ala). However,the two enzymes were fermented separately and cannot be immobilized.In this study,AspAT and PanD was produced in one fermentation process by coexpression in E.coli,and the enzymes can be reused by immobilization.Activity of the recombinant cell was 85.4 U/g,and the immobilization yield reached 64.2%.The optimum reaction temperature and pH of the immobilized cells was 45℃and 7.5,respectively.β-Ala was produced by the immobilized cells at high fumaric acid concentration of 300 g/L.The immobilized cells can be used continuously for 12 times.Based on these results,the fermentation process can be simplified,and the cost ofβ-Ala production can be further reduced.
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