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Determination of hyperoside in stems and leaves of Rosa davurica Pall. by HPLC
Pages: 575-576,579
Year: Issue:  10
Journal: Qilu Pharmaceutical Affairs

Keyword:  山刺玫金丝桃苷含量测定高效液相色谱;
Abstract: 目的 采用高效液相色谱(HPLC)法建立山刺玫茎叶中金丝桃苷的含量测定方法,以期为山刺玫的质量标准提供依据.方法 以乙腈-甲醇-水(10:10:80,V:V:V)为流动相,Microsorb C18为固定相.结果 金丝桃苷的线性范围0.012~0.12 mg?mL-1;山刺玫茎叶中金丝桃苷的含量为1.53~2.20 mg?g-1.结论 金丝桃苷为山刺玫茎叶中的特征黄酮类活性成分,该测定方法的建立有助于山刺玫的全面质量控制.
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