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Determination of quercetin and kaempferol in tartary buckwheat tea and fingerprint analysis
Author(s): 
Pages: 646-648,653
Year: Issue:  11
Journal: Qilu Pharmaceutical Affairs

Keyword:  苦荞茶高效液相色谱法指纹图谱;
Abstract: 目的 建立苦荞茶高效液相色谱(HPLC)指纹图谱,并测定不同批次苦荞茶中槲皮素、山柰素的含量,为苦荞茶的质量控制提供参考方法.方法 色谱柱为Agilent C18(4.6 mm×250 mm,5μm);流动相:乙腈-0.2%磷酸梯度洗脱,检测波长260 nm,柱温28℃,流速1 mL?min-1,进样量10μL.结果 黑苦荞茶中槲皮素的含量总体高于黄苦荞茶,12批苦荞茶指纹图谱之间的相似度为0.988~1.000.结论 该方法简便、准确、重复性好,可用于苦荞茶的质量控制.
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