The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
Determination of the serum NTx in toxicity of YKY-011 to cynomolgus monkey via subcutaneous injection
Author(s): 
Pages: 16-18
Year: Issue:  1
Journal: Qilu Pharmaceutical Affairs

Keyword:  酶联免疫吸附测定法I型胶原交联氨基末端肽骨质疏松食蟹猴血清;
Abstract: 目的 建立食蟹猴血清Ⅰ型胶原交联氨基末端肽(NTx)的含量检测方法,用以评估YKY-011重复给予食蟹猴毒性试验中I型胶原交联氨基末端肽的含量变化.方法 采用竞争酶联免疫吸附测定(ELISA)法检测,验证考察了检测方法的线性范围、精密度和准确性,并对YKY-011重复给药毒性试验中食蟹猴的血清I型胶原交联氨基末端肽含量进行了检测.结果 建立并验证了灵敏度高、稳定性良好的检测猴血清中I型胶原交联氨基末端肽的酶联免疫吸附测定方法;食蟹猴连续皮下注射YKY011在给药2周后较给药前血清I型胶原交联氨基末端肽开始下降,且与同剂量市售对照组下降幅度相当.结论 该酶联免疫吸附测定方法可用于食蟹猴血清Ⅰ型胶原交联氨基末端肽的检测,YKY-011可有效降低食蟹猴血清中I型胶原交联氨基末端肽水平,并与市售对照品降低幅度类似.
Related Articles
No related articles found