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Study on L-isoleucine analysis by high performance liquid chromatography
Author(s): 
Pages: 220-223
Year: Issue:  4
Journal: FAJIAO KEJI TONGXUN

Keyword:  异亮氨酸高效液相色谱OPA自动衍生手动衍生;
Abstract: L-异亮氨酸在紫外光区无吸收峰,需将其进行衍生后才能测定.通过对比两种柱前衍生方法,建立高效液相色谱法柱前衍生检测L-异亮氨酸质量分数的方法.OPA自动衍生法检测耗时15 min,R=1,加标回收率99%~100.1%,RSD<1.0%;PITC手动衍生法检测耗时30 min,加标回收率96%~98%,RSD<1%.O PA自动衍生法在检测的时效性和准确性方面均具有明显优势,可用来高效地检测L-异亮氨酸的质量分数,在生物发酵、饲料添加剂、食品添加剂和制药等领域具有较高的应用价值.
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