Author(s): WANG Zhuo, LIU Huaiying
Pages: 683-686
Year: 2016 Issue:  12
Journal: Qilu Pharmaceutical Affairs
Keyword:  Bevacizumab; M2 macrophage; Inflammatory cytokines; Tumor cell migration;
Abstract: Objective To study the relationship between bevacizumab,macrophages,and tumor cells in the tumor mi-croenvironment,and find the potential mechanism of invalid treatment of bevacizumab in HCC(hepatic cell carcinoma). Methods The phenotype of macrophages in HCC was determined by immunohistochemistry.M2 macrophages and tumor cells were cocultured and bevacizumab was added.The release of inflammatory cytokines and vascular growth factor were de-tected by ELISA.The impact of supernant from coculture system on tumor cell migration was detected.ResuIts Most macro-phages in HCC microenvironment had M2 characteristics.Bevacizumab increased the level of inflammatory cytokines and vascular endothelial growth factor(VEGF)significantly in the M2 macrophages and tumor cells coculture system.Supernant from M2 macrophages and tumor cells coculture system promoted tumor cell migration.ConcIusion Bevacizumab can acti-vate M2 macrophage and promote cancer metastasis.