Preparation of oxaliplatin liposome and in vitro drug - release
Author(s): WANG Jianzhao, Li Yanan, XIONG Yerong, SHEN Yan, TU Jiasheng, SUN Chunmeng
Pages: 217-221,225
Year: 2016 Issue: 4
Journal: Qilu Pharmaceutical Affairs
Keyword: Oxaliplatin; Liposome; Formulation screening; In vitro release; HPLC; AAS;
Abstract: Objective To prepare oxaliplatin liposomes with high encapsulation efficiency,and to investigate the in vitro properties of them. Methods Compare oxaliplatin liposomes prepared by several methods,and determine the best pre-scription through the single factor experiment and orthogonal experiment. In the experiment HPLC was used to determine the encapsulation efficiency,and Zetaplus Laser Particle Size Analyzer was used to determine the particle size and zeta potential of liposomes. In addition,both HPLC method and AAS method was used to investigate the in vitro release of the liposomes. Results Compared with thin - film dispersion method and pH gradient method,liposomes prepared by reverse phase evap-oration method presented higher encapsulation efficiency. The best prescription determined by formulation screening was:the ratio of drug to lipid with 1∶7. 5,the ratio of SPC to CH with 2∶1,the ultrasonic power with 195 W and the ultrasonic time with 3 min. In vitro drug release study indicated that the accumulative releasing degree of oxaliplatin liposomes in 24 h was 25. 0% ,determined by HPLC,and 33. 6% ,determined by AAS. Conclusion Liposomes prepared by the reverse phase e-vaporation method were stable enough and assume high encapsulation efficiency and showed the capability of sustained - re-leasing.
Pages: 217-221,225
Year: 2016 Issue: 4
Journal: Qilu Pharmaceutical Affairs
Keyword: Oxaliplatin; Liposome; Formulation screening; In vitro release; HPLC; AAS;
Abstract: Objective To prepare oxaliplatin liposomes with high encapsulation efficiency,and to investigate the in vitro properties of them. Methods Compare oxaliplatin liposomes prepared by several methods,and determine the best pre-scription through the single factor experiment and orthogonal experiment. In the experiment HPLC was used to determine the encapsulation efficiency,and Zetaplus Laser Particle Size Analyzer was used to determine the particle size and zeta potential of liposomes. In addition,both HPLC method and AAS method was used to investigate the in vitro release of the liposomes. Results Compared with thin - film dispersion method and pH gradient method,liposomes prepared by reverse phase evap-oration method presented higher encapsulation efficiency. The best prescription determined by formulation screening was:the ratio of drug to lipid with 1∶7. 5,the ratio of SPC to CH with 2∶1,the ultrasonic power with 195 W and the ultrasonic time with 3 min. In vitro drug release study indicated that the accumulative releasing degree of oxaliplatin liposomes in 24 h was 25. 0% ,determined by HPLC,and 33. 6% ,determined by AAS. Conclusion Liposomes prepared by the reverse phase e-vaporation method were stable enough and assume high encapsulation efficiency and showed the capability of sustained - re-leasing.
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