Author(s): HE Haiyan, ZHANG Ye, LIU Hongming
Pages: 214-216
Year: 2016 Issue:  4
Journal: Qilu Pharmaceutical Affairs
Keyword:  Xiaoer Jiebiao Granules; HPLC; Chlorogenic acid; Caffeic acid; Puerarin; Daidzin;
Abstract: Objective To simultaneous determine the contents of chlorogenic acid,caffeic acid,puerarin,daidzin in Xi-aoer Jiebiao Granules by HPLC. Methods Agilent Zorbax XDB - C18 column(4. 6 mm × 250 mm,5 μm)was adopted with acetonitrile - 0. 1% phosphoric acid as the mobile phases by gradient elution. The column temperature was 40 ℃ . The de-tection wavelength was changed. Results The linear ranges of chlorogenic acid,caffeic acid,puerarin,daidzin were 0. 051 46 ~ 1. 029 2 μg,0. 024 70 ~ 0. 494 0 μg,0. 050 57 ~ 1. 011 4 μg,0. 008 292 ~ 0. 165 8 μg,respectively. The aver-age recoveries were between 98. 77% ~ 99. 98% with RSDs less than 2. 00% . Conclusion The method was accurate,sen-sitive,reproducible,it can be used for the overall assessment of the quality of Xiaoer Jiebiao Granules.