Study on mechanism of Tibetan Gentianopsispaludosa(Hook.f.)Ma.extraction(GPE) inducing colon cancer cells apoptosis by NF -κB signaling pathway
Author(s): LU Nianhua, ZHAO Huiqiao, CHEN Zhengjun, JING Ming, ZHANG Yanxia
Pages: 129-132
Year: 2016 Issue: 3
Journal: Qilu Pharmaceutical Affairs
Keyword: Gentiano psispaludosa(Hook.f.)Ma; Colon cancer; Cell apoptosis; NF -κB signaling pathway;
Abstract: Objective To explore the mechanism of GPE inducingapoptosis of colon SW480 cancer cells by NF -κB signaling pathway.Methods Colon SW480 cancer cells were treated with different concentrations of GPE.JC -1 staining was used to detect changes of mitochondrial membrane potential and hoechst staining was used to determine whether the ex-traction can induce apoptosis in colon SW480 cancer cells or not.Simultaneously,expression of NF -κB protein was detec-ted to clear the mechanism of NF -κB signaling pathway in coloniccarcinoma.Results The expression of NF -κB in SW480 cells became depress with treatment of certain concentration of GPE,and the phenomenons of staining showed that GPE had a potential ability to result in cancer cell apoptosis.Conclusion GPE can induce apoptosis of tumor cells in vitro,and the mechanism might be partly related to NF -κB signaling pathway.
Pages: 129-132
Year: 2016 Issue: 3
Journal: Qilu Pharmaceutical Affairs
Keyword: Gentiano psispaludosa(Hook.f.)Ma; Colon cancer; Cell apoptosis; NF -κB signaling pathway;
Abstract: Objective To explore the mechanism of GPE inducingapoptosis of colon SW480 cancer cells by NF -κB signaling pathway.Methods Colon SW480 cancer cells were treated with different concentrations of GPE.JC -1 staining was used to detect changes of mitochondrial membrane potential and hoechst staining was used to determine whether the ex-traction can induce apoptosis in colon SW480 cancer cells or not.Simultaneously,expression of NF -κB protein was detec-ted to clear the mechanism of NF -κB signaling pathway in coloniccarcinoma.Results The expression of NF -κB in SW480 cells became depress with treatment of certain concentration of GPE,and the phenomenons of staining showed that GPE had a potential ability to result in cancer cell apoptosis.Conclusion GPE can induce apoptosis of tumor cells in vitro,and the mechanism might be partly related to NF -κB signaling pathway.
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