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Determination of three saponins in Cortex Eucommia Zhuanggu Capsules by HPLC
Pages: 148-150,178
Year: Issue:  3
Journal: Qilu Pharmaceutical Affairs

Keyword:  Cortex Eucommia Zhuanggu CapsulesNotoginsenoside R1Ginsenoside Rg1Ginsenoside ReHPLC;
Abstract: Objective To establish an HPLC method for the simultaneous determination of notoginsenoside R1 (C47 H80 O18 ),ginsenoside Rg1 (C42 H72 O14 )and ginsenoside Re(C48 H82 O18 )in Cortex Eucommia Zhuanggu Capsules were identi-fied,in order to improve the quality control of Cortex Eucommia Zhuanggu Capsules.Methods Capcell Pak C18 column (4.6 mm ×250 mm,5 μm)was adopted to separate the three saponins by acetonitrile -1% phosphoric acid(18.8∶81.2) as mobile phase with detection wavelength of UV detector at 203 nm.The flow rate was 1.5 mL·min -1 and the column temperature was 30 ℃.Results The linear ranges of notoginsenoside R1 ,ginsenoside Rg1 and ginsenoside Re were 4.99 ~99.7 μg·mL -1 ,5.76 ~115.1 μg·mL -1 and 54.05 ~1 081 μg·mL -1 ,respectively.Conclusion This method was convenient,accuracy,high sensitivity and good repetition,it can reflect the qualities of medical components in preparations and can be used for quality control of Cortex Eucommia Zhuanggu Capsules.
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