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Simultaneous determination of three saponins in Shuxue Tongmai Capsules by HPLC
Author(s): 
Pages: 137-138,141
Year: Issue:  3
Journal: Qilu Pharmaceutical Affairs

Keyword:  疏血通脉胶囊高效液相色谱法人参皂苷Rg1人参皂苷Rb1三七皂苷R1;
Abstract: 目的 建立测定疏血通脉胶囊中三七有效成分含量的方法.方法 采用高效液相色谱法,以Kromasil C18(4.6 mm × 250 mm,5μm)为色谱柱,以乙腈-水为流动相进行梯度洗脱,流速1.0 mL· min-1,检测波长203nm.结果 人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1进样量分别在1.02 ~ 10.20 μg、0.95 ~9.50 μg和0.35~3.50 μg范围内与各自峰面积积分值呈良好线性关系(r=0.999 9),平均回收率分别为100.40%、98.90%和99.61%,RSD分别为1.71%、1.98%和1.69%(n=6).结论 所采用的方法操作简单,可靠,准确,可作为疏血通脉胶囊的质量控制方法.
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