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The research on isolation, identification and purification, and optimization of counting medium component of Rhodopseudomonas palustris
Author(s): 
Pages: 322-326
Year: Issue:  3
Journal: Journal of Southwest University for Nationalities(Natrual Science Edition)

Keyword:  沼泽红假单胞菌光合菌分离与纯化;
Abstract: 目的:通过分离鉴、纯化与鉴定菌株,然后按沼泽红假单胞菌所需的营养组分,优化计数培养基的配方,为生产中的计数提供试验数据.方法:待分离的菌落接种于培养基,然后进行反复的纯化,最后观察菌落的形态,按照细菌鉴定手册鉴定;按所需碳源、氮源、磷酸盐、酵母膏等因素进进行正交设计,优化出最佳的培养基配方.结论:分离与纯化得到一株红红螺菌科沼泽红假单胞菌;通过优化得到最优及验证试验得到最佳的计数培养基配方为:氯化铵1.5 g/L,乙酸钠1.5 g/L磷酸氢二钾0.4g/L,氯化钠1.0g/L,硫酸镁0.3g/L,酵母膏2.0g/L,琼脂14.0g/L.该配方能够对沼泽红假单胞菌准确及简捷的计数.
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