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Study on identification method for Flos lonicerae japonicae and Flos lonicerae
Author(s): 
Pages: 69-71
Year: Issue:  2
Journal: JOURNAL OF PHARMACEUTICAL RESEARCH

Keyword:  金银花山银花灰毡毛忍冬皂苷乙高效液相色谱法;
Abstract: 目的 建立高效液相色谱-蒸发光散射检测法(HPLC-ELSD)定性鉴别方法,以区分金银花与山银花及其制剂.方法 以灰毡毛忍冬皂苷乙为指标成分,十八烷基硅烷键合硅胶为填充剂;乙腈-0.4%醋酸为流动相,梯度洗脱,流速为1.0 mL·min-1,用蒸发光散射检测器.结果 金银花及其制剂未检出灰毡毛忍冬皂苷乙,山银花及其制剂均检出.结论 建立的HPLC-ELSD定性鉴别方法,可用于金银花及其提取物和制剂中非法添加山银花的检查,为规范金银花药材市场提供技术支持.
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