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Study on the Protein Diffusion Behavior in the Poly(Acrylamide-co-Acrylic Acid) Copolymer by FRAP Technique
Author(s): 
Pages: 336-343
Year: Issue:  5
Journal: Imaging Science and Photochemistry

Keyword:  聚丙烯酰胺-丙烯酸荧光漂白恢复技术荧光标记蛋白质扩散系数;
Abstract: 药物分子从药物载体中的释放行为与载体的结构有密切关系.本实验中采用丙烯酰胺和丙烯酸等材料,运用水相沉淀的方法,制备了4种不同单体配比的聚丙烯酰胺-丙烯酸(P(Am-co-Ac)共聚物水凝胶.运用红外分析方法对P (Am-co-Ac)组成进行表征.使用荧光漂白恢复法(FRAP,fluorescence recovery after photobleaching)观察荧光素FITC标记的牛血清白蛋白(...
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