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li zi jiao huan shu zhi fen li chun hua gu zuo gan zuo de yan jiu
Author(s): 
Pages: 20-22
Year: Issue:  4
Journal: FAJIAO KEJI TONGXUN

Keyword:  树脂GSH洗脱条件;
Abstract: 研究001×7阳离子交换树脂分离纯化谷胱甘肽的工艺条件,考察了001×7阳离子交换树脂对GSH的静态吸附量,以及洗脱时,洗脱液,洗脱浓度,洗脱流速等对分离纯化产品GSH的影响.确定其工艺流程;最适上柱pH为2.5,洗脱液NaCl溶液最适洗脱浓度为1.2%,洗脱流速为2.0ml/min;收集洗脱液,用紫外分先光度法检测其GSH的浓度,回收率为75%,纯度相对提高60%.
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