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Introduction of Shirley Clift modified-TG method for extracting genomic DNA and its application in Gene-Trap
Author(s): 
Pages: 1155-1156
Year: Issue:  9
Journal: ZHEJIANG CLINICAL MEDICAL JOURNAL

Keyword:  小鼠基因组DNADNA提取基因型鉴定;
Abstract: 目的:介绍一种简单、快速、高效同时从数十、甚至上百个小鼠尾巴中提取基因组DNA的方法.方法 Shirley Clift modified-TG 小鼠Gene-Trap DNA提取法.结果 使用该法从小鼠尾巴中获取的基因组DNA的数量、纯度完全适用各种实验,如在C3H/HCJ-Mgrn1md转基因小鼠杂交C57BL/6小鼠Mahogunin基因变异传代培殖实验中,应用鼠尾抽提的DNA 进行Gene-trap.类推该法到小鼠其它组织如肝、肾、肌肉和心脏,也同样得到了高质量的基因组DNA.结论 该技术可以连续、高效、快速、批量从小鼠组织中提取基因组DNA,为国内利用小鼠进行基因遗传疾病的研究提供了基础方法.
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