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Determination of Chlorogenic acid in Honeysuckle Stem by HPLC
Author(s): 
Pages: 26-27
Year: Issue:  1
Journal: QILU PHARMACEUTICAL AFFAIRS

Keyword:  忍冬藤绿原酸高效液相色谱法含量测定;
Abstract: 目的 建立忍冬藤中绿原酸的含量测定方法.方法 采用高效液相色谱法,色谱柱:Thermo Hypersil BDS (4.6×250mm,5μm);检测波长: 327nm;流动相:乙腈-0.4%磷酸溶液(10∶90).结果 绿原酸进样量在0.1656~0.9936μg范围内与峰面积呈良好的线性关系,r=0.9999.平均加样回收率为98.79%,RSD为1.07%(n=5) .结论 本方法准确、简便、快速,适合于该药材的质量分析检验.
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